Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

doi: 10.12659/MSM.912109

Figure Lengend Snippet: POU5F1B controls the expression of OCT4. ( A ) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the mRNA levels of OCT4 after transfected with si-POU5F1B or si-NC. The data are represented as the mean ±SD. * P<0.05. ( B ) Western blot analysis was performed to determine the OCT4 protein expression after transfection with si-POU5F1B or si-NC.

Article Snippet: The membranes were blocked with 5% dried skimmed milk powder for an hour and incubated in primary antibodies to OCT4 (Boster, Wuhan, China) at 4°C overnight and GAPDH (Beyotime, Haimen, China) at 4°C overnight.

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Western Blot

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 protein in pancreatic cancer tissues (magnification, ×200). Pancreatic cancer tissues and ANCT were immunohistochemically stained with an anti-OCT4 antibody and classified as (−) and (+). (A) Positive expression in pancreatic cancer. (B) Negative expression in pancreatic cancer. (C) Positive expression in ANCT. (D) Negative expression in ANCT. Positive immunostaining of OCT4 was mainly localized in the nucleus of the tumor and ANCT cells. OCT4, octamer-binding transcription factor 4; ANCT, adjacent non-cancer tissues.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Staining, Immunostaining, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 protein in pancreatic cancer tissues.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 in pancreatic cancer cells with different degrees of differentiation. The expression of OCT4 in pancreatic cancer cells with different degrees of differentiation (Bxpc3, Panc-1 and Mia PaCa-2) was examined by (A) real-time PCR and (B and C) western blot assays, of which OCT4 was highly expressed in the Panc-1 cell line compared with the other two cell lines (P<0.01). OCT4, octamer binding transcription factor 4.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Correlation of OCT4 expression with the clinicopathological characteristics of patients with pancreatic cancer.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on the expression of AKT in pancreatic cancer cells. After pancreatic cancer cells were transfected with the Lv-shOCT4 for 24 h, the expression levels of OCT4 and AKT were detected by (A and B) real-time PCR and (C–F) western blot analysis. The expression of OCT4 and AKT was significantly decreased in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; CON, control vector; NC, negative control vector.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on cell proliferation. (A) Cell proliferative activity, indicated by MTT assay, markedly decreased in a time-dependent manner in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). (B and C) Endogenous expression of PCNA, indicated by western blot analysis, was significantly decreased in the Lv-shOCT4 group compared with the NC and CON groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; PCNA, proliferating cell nuclear antigen; CON, control vector; NC, negative control vector.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Activity Assay, MTT Assay, Expressing, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on cell invasion (magnification, ×200). (A and B) Cell invasive potential, indicated by Transwell assay, was markedly weakened in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). (C and D) Endogenous expression of MMP-2, indicated by western blot analysis, was significantly decreased in the Lv-shOCT4 group compared with the NC and CON groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; CON, control vector; NC, negative control vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP-2, matrix metalloproteinase-2.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Transwell Assay, Expressing, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Oncology Letters

Article Title: S100A16 up-regulates Oct4 and Nanog expression in cancer stem-like cells of Yumoto human cervical carcinoma cells

doi: 10.3892/ol.2018.8568

Figure Lengend Snippet: The expression levels of Oct4, Nanog, and S100A16 in the sphere formation of Yumoto cells. The cells were cultured under the non-adherence and serum-free culture conditions of the sphere formation assay for 3 days. (A) Total RNA was extracted, and the expressions of Oct4, Nanog, and S100A16 were evaluated by reverse transcription-polymerase chain reaction. (B) The cell lysates were prepared from these cells, and the expressions of Oct4, Nanog, and S100A16 were detected by immunoblotting using antibodies against Oct4, Nanog, and S100A16. Oct4, octamer-binding transcription factor 4; Nanog, homeobox protein NANOG.

Article Snippet: The membranes were washed in Tris-buffered saline with Tween 20 (TBS-T, composed of 10 mM Tris-HCl, pH 8, 150 mM NaCl, and 0.05% Tween 20), blocked for 1 h at room temperature with 5% nonfat milk in TBS-T, and probed overnight at 4°C with anti-S100A16 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), anti-Oct4 (Bioworld Technology, Inc., St. Louis Park, MN, USA), anti-Nanog (Abcam, Cambridge, UK), anti-p53 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and anti-GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA) antibodies.

Techniques: Expressing, Cell Culture, Tube Formation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Binding Assay

Journal: Oncology Letters

Article Title: S100A16 up-regulates Oct4 and Nanog expression in cancer stem-like cells of Yumoto human cervical carcinoma cells

doi: 10.3892/ol.2018.8568

Figure Lengend Snippet: Effect of S100A16-targeting siRNA on the expression of Oct4, Nanog, and p53 in the sphere formation of Yumoto cells. The cells were transfected with S100A16-targeting or control siRNA. After these cells were incubated for 24 h, these cells were cultured under the conditions of the sphere formation assay for 3 days. (A) Total RNA was extracted, and the expression levels of Oct4, Nanog, p53, and S100A16 were evaluated by reverse transcription-polymerase chain reaction. (B) The cell lysates were prepared from these cells, and the expression levels of Oct4 Nanog, p53, and S100A16 were detected by immunoblotting using antibodies against Oct4, Nanog, p53, and S100A16. Oct4, octamer-binding transcription factor 4; Nanog, homeobox protein NANOG; siRNA, short interfering RNA.

Article Snippet: The membranes were washed in Tris-buffered saline with Tween 20 (TBS-T, composed of 10 mM Tris-HCl, pH 8, 150 mM NaCl, and 0.05% Tween 20), blocked for 1 h at room temperature with 5% nonfat milk in TBS-T, and probed overnight at 4°C with anti-S100A16 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), anti-Oct4 (Bioworld Technology, Inc., St. Louis Park, MN, USA), anti-Nanog (Abcam, Cambridge, UK), anti-p53 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and anti-GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA) antibodies.

Techniques: Expressing, Transfection, Incubation, Cell Culture, Tube Formation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Binding Assay, Small Interfering RNA

Journal: Oncology Letters

Article Title: S100A16 up-regulates Oct4 and Nanog expression in cancer stem-like cells of Yumoto human cervical carcinoma cells

doi: 10.3892/ol.2018.8568

Figure Lengend Snippet: (A) Effect of proteasome inhibition on the expressions of Oct4, Nanog, p53, and S100A16 in the sphere formation of Yumoto cells. After the cells were cultured under the conditions of the sphere formation assay for 3 days, these cells were treated with proteasome inhibitor lactacystin (10 µM) under the conditions of the sphere formation assay for 24 h. The cell lysates were prepared from these cells, and the expression levels of Oct4, Nanog, p53, and S100A16 were detected by immunoblotting using antibodies against Oct4, Nanog, p53, and S100A16. (B) Potential schematic diagram of the effect of S100A16 on the expression of Oct4 and Nanog expression in CSCs of Yumoto human cervical carcinoma cells. Oct4, octamer-binding transcription factor 4; Nanog, homeobox protein NANOG.

Article Snippet: The membranes were washed in Tris-buffered saline with Tween 20 (TBS-T, composed of 10 mM Tris-HCl, pH 8, 150 mM NaCl, and 0.05% Tween 20), blocked for 1 h at room temperature with 5% nonfat milk in TBS-T, and probed overnight at 4°C with anti-S100A16 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), anti-Oct4 (Bioworld Technology, Inc., St. Louis Park, MN, USA), anti-Nanog (Abcam, Cambridge, UK), anti-p53 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and anti-GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA) antibodies.

Techniques: Inhibition, Cell Culture, Tube Formation Assay, Expressing, Western Blot, Binding Assay

Journal: Bioengineering

Article Title: Liquid Helium Enhanced Vitrification Efficiency of Human Bone-Derived Mesenchymal Stem Cells and Human Embryonic Stem Cells

doi: 10.3390/bioengineering8110162

Figure Lengend Snippet: Typical antibodies to confirm hBMSCs and hESCs.

Article Snippet: hESCs , Intranuclear antigens , Rabbit anti-Oct-4 (Signalway Antibody) (#49129) , Mouse anti-rabbit IgG FITC (Bioss Antibodies) (bs-0295M-FITC).

Techniques: